Review



t vector pmd20  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    TaKaRa t vector pmd20
    T Vector Pmd20, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t+vector+pmd20/T-Vector+pMD+20/pm42809396-313-11-14
    Average 97 stars, based on 1432 article reviews
    t vector pmd20 - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    other:

    Article Title: The non-coding facet of Pink1 mRNA regulates mitochondria homeostasis
    Article Snippet: Genomic DNA was extracted and treated with EpiArt DNA Methylation Bisulfite Kit (Vazyme, EM101, China) according to the manufacturer’s instructions.

    Purification:

    Article Title: A reverse transcription-quantitative polymerase chain reaction system for evaluating intestinal butyrate production by fecal bacteria.
    Article Snippet: 27 . (Beckman Coulter Inc., Brea, CA, USA), and the DNA concentration was calculated based on the measured OD260 value. .. The purified DNA product was ligated with a T-Vector pMD20 (Takara, Shiga, Japan, #3270) using the DNA Ligation Kit ver. .. 2.1 (Takara, #6022) and transformed into XL10-Gold Ultracompetent Cells (Agilent, Santa Clara, CA, USA, #200314).

    Article Title: A reverse transcription-quantitative polymerase chain reaction system for evaluating intestinal butyrate production by fecal bacteria
    Article Snippet: OD 260 was measured using a DU730 spectrophotometer (Beckman Coulter Inc., Brea, CA, USA), and the DNA concentration was calculated based on the measured OD 260 value. .. The purified DNA product was ligated with a T-Vector pMD20 (Takara, Shiga, Japan, #3270) using the DNA Ligation Kit ver. .. 2.1 (Takara, #6022) and transformed into XL10-Gold Ultracompetent Cells (Agilent, Santa Clara, CA, USA, #200314).

    Article Title: A minor muscle stem cell population functions as metabolic Trojan horse fueling systemic aging by succinate induced epigenetic reprogramming
    Article Snippet: Genomic DNA was extracted and treated with EpiArt DNA Methylation Bisulfite Kit (Vazyme, EM101, China) according to the manufacturer’s instructions. .. Bisulfite-treated DNA was subjected to nested PCR amplification, then purified with the Gel Extraction Kit (TianGen, DP204, China) and cloned into T-Vector pMD20 (Takara, 3270, Japan). ..

    DNA Ligation:

    Article Title: A reverse transcription-quantitative polymerase chain reaction system for evaluating intestinal butyrate production by fecal bacteria.
    Article Snippet: 27 . (Beckman Coulter Inc., Brea, CA, USA), and the DNA concentration was calculated based on the measured OD260 value. .. The purified DNA product was ligated with a T-Vector pMD20 (Takara, Shiga, Japan, #3270) using the DNA Ligation Kit ver. .. 2.1 (Takara, #6022) and transformed into XL10-Gold Ultracompetent Cells (Agilent, Santa Clara, CA, USA, #200314).

    Article Title: A reverse transcription-quantitative polymerase chain reaction system for evaluating intestinal butyrate production by fecal bacteria
    Article Snippet: OD 260 was measured using a DU730 spectrophotometer (Beckman Coulter Inc., Brea, CA, USA), and the DNA concentration was calculated based on the measured OD 260 value. .. The purified DNA product was ligated with a T-Vector pMD20 (Takara, Shiga, Japan, #3270) using the DNA Ligation Kit ver. .. 2.1 (Takara, #6022) and transformed into XL10-Gold Ultracompetent Cells (Agilent, Santa Clara, CA, USA, #200314).

    Article Title: A Water-Saving Drought Survival Phenotype in a Wheat TILLING Mutant Involves Survival-Biased Metabolic and Phosphorylation Reprogramming.
    Article Snippet: .. For TA cloning, 0.5 μL of T‐Vector pMD20 (Takara Bio Inc., Shiga, Japan; Cat. No. 3270), 0.5 μL of PCR product, and 1.5 μL of ultrapure water were mixed in a 1.5 mL tube, followed by the addition of 2.5 μL of DNA Ligation Mix (Takara Bio; Cat. No. 6023) and incubation at 16°C for 30 min. .. The entire ligation mixture was transformed into 50 μL of DH5α competent cells (Takara Bio, Cat. No. 9057) and spread on LB agar plates containing 100 μg/mL ampicillin.

    Polymerase Chain Reaction:

    Article Title: TCR-mTORC1 signaling promotes epigenetic stabilization of Foxp3 expression in regulatory T cells via TET protein translation.
    Article Snippet: .. PCR products were cloned into pGEM- T Easy Vector (Promega) or T- Vector pMD20 (Takara) and transformed into E. coli. .. Plasmids from resulting colonies were amplified using the Illustra TempliPhi DNA Amplification Kit (GE Healthcare).

    Article Title: A Water-Saving Drought Survival Phenotype in a Wheat TILLING Mutant Involves Survival-Biased Metabolic and Phosphorylation Reprogramming.
    Article Snippet: .. For TA cloning, 0.5 μL of T‐Vector pMD20 (Takara Bio Inc., Shiga, Japan; Cat. No. 3270), 0.5 μL of PCR product, and 1.5 μL of ultrapure water were mixed in a 1.5 mL tube, followed by the addition of 2.5 μL of DNA Ligation Mix (Takara Bio; Cat. No. 6023) and incubation at 16°C for 30 min. .. The entire ligation mixture was transformed into 50 μL of DH5α competent cells (Takara Bio, Cat. No. 9057) and spread on LB agar plates containing 100 μg/mL ampicillin.

    Clone Assay:

    Article Title: TCR-mTORC1 signaling promotes epigenetic stabilization of Foxp3 expression in regulatory T cells via TET protein translation.
    Article Snippet: .. PCR products were cloned into pGEM- T Easy Vector (Promega) or T- Vector pMD20 (Takara) and transformed into E. coli. .. Plasmids from resulting colonies were amplified using the Illustra TempliPhi DNA Amplification Kit (GE Healthcare).

    Article Title: A minor muscle stem cell population functions as metabolic Trojan horse fueling systemic aging by succinate induced epigenetic reprogramming
    Article Snippet: Genomic DNA was extracted and treated with EpiArt DNA Methylation Bisulfite Kit (Vazyme, EM101, China) according to the manufacturer’s instructions. .. Bisulfite-treated DNA was subjected to nested PCR amplification, then purified with the Gel Extraction Kit (TianGen, DP204, China) and cloned into T-Vector pMD20 (Takara, 3270, Japan). ..

    Transformation Assay:

    Article Title: TCR-mTORC1 signaling promotes epigenetic stabilization of Foxp3 expression in regulatory T cells via TET protein translation.
    Article Snippet: .. PCR products were cloned into pGEM- T Easy Vector (Promega) or T- Vector pMD20 (Takara) and transformed into E. coli. .. Plasmids from resulting colonies were amplified using the Illustra TempliPhi DNA Amplification Kit (GE Healthcare).

    Nested PCR:

    Article Title: A minor muscle stem cell population functions as metabolic Trojan horse fueling systemic aging by succinate induced epigenetic reprogramming
    Article Snippet: Genomic DNA was extracted and treated with EpiArt DNA Methylation Bisulfite Kit (Vazyme, EM101, China) according to the manufacturer’s instructions. .. Bisulfite-treated DNA was subjected to nested PCR amplification, then purified with the Gel Extraction Kit (TianGen, DP204, China) and cloned into T-Vector pMD20 (Takara, 3270, Japan). ..

    Amplification:

    Article Title: A minor muscle stem cell population functions as metabolic Trojan horse fueling systemic aging by succinate induced epigenetic reprogramming
    Article Snippet: Genomic DNA was extracted and treated with EpiArt DNA Methylation Bisulfite Kit (Vazyme, EM101, China) according to the manufacturer’s instructions. .. Bisulfite-treated DNA was subjected to nested PCR amplification, then purified with the Gel Extraction Kit (TianGen, DP204, China) and cloned into T-Vector pMD20 (Takara, 3270, Japan). ..

    Article Title: miR-765 induces angiogenesis by inhibiting dipeptidyl peptidase 4 and increasing fibroblast growth factor 2
    Article Snippet: The miRNA expression levels were analyzed using 3D-Gene® miRNA oligochips (Toray Industries, Inc., Kamakura, Kanagawa, Japan) (GEO accession: GSE232178 and Supplementary Data). .. To construct the plasmids expressing the 12 miRNAs, we amplified the 293 T cell genome with Ex Taq (TaKaRa Bio, Inc.) and primers and ligated the amplified DNA to a T-vector pMD20 (#3270, TaKaRa Bio Inc.). ..

    Gel Extraction:

    Article Title: A minor muscle stem cell population functions as metabolic Trojan horse fueling systemic aging by succinate induced epigenetic reprogramming
    Article Snippet: Genomic DNA was extracted and treated with EpiArt DNA Methylation Bisulfite Kit (Vazyme, EM101, China) according to the manufacturer’s instructions. .. Bisulfite-treated DNA was subjected to nested PCR amplification, then purified with the Gel Extraction Kit (TianGen, DP204, China) and cloned into T-Vector pMD20 (Takara, 3270, Japan). ..

    Construct:

    Article Title: miR-765 induces angiogenesis by inhibiting dipeptidyl peptidase 4 and increasing fibroblast growth factor 2
    Article Snippet: The miRNA expression levels were analyzed using 3D-Gene® miRNA oligochips (Toray Industries, Inc., Kamakura, Kanagawa, Japan) (GEO accession: GSE232178 and Supplementary Data). .. To construct the plasmids expressing the 12 miRNAs, we amplified the 293 T cell genome with Ex Taq (TaKaRa Bio, Inc.) and primers and ligated the amplified DNA to a T-vector pMD20 (#3270, TaKaRa Bio Inc.). ..

    Expressing:

    Article Title: miR-765 induces angiogenesis by inhibiting dipeptidyl peptidase 4 and increasing fibroblast growth factor 2
    Article Snippet: The miRNA expression levels were analyzed using 3D-Gene® miRNA oligochips (Toray Industries, Inc., Kamakura, Kanagawa, Japan) (GEO accession: GSE232178 and Supplementary Data). .. To construct the plasmids expressing the 12 miRNAs, we amplified the 293 T cell genome with Ex Taq (TaKaRa Bio, Inc.) and primers and ligated the amplified DNA to a T-vector pMD20 (#3270, TaKaRa Bio Inc.). ..

    Sequencing:

    Article Title: Threonyl-tRNA Synthetase Is the Target of Borrelidin Insecticidal Activity against Silkworms.
    Article Snippet: We explore borrelidin and semisynthetic derivatives as promising pesticide candidates targeting threonyl-tRNA synthetase (ThrRS).. Enzymatic inhibition assays and pull-down assays supported ThrRS as the target of borrelidins for insecticidal activity.. Interestingly, derivative 6 showed weak in vitro inhibition but comparable in vivo insecticidal activity to borrelidin.

    TA Cloning:

    Article Title: A Water-Saving Drought Survival Phenotype in a Wheat TILLING Mutant Involves Survival-Biased Metabolic and Phosphorylation Reprogramming.
    Article Snippet: .. For TA cloning, 0.5 μL of T‐Vector pMD20 (Takara Bio Inc., Shiga, Japan; Cat. No. 3270), 0.5 μL of PCR product, and 1.5 μL of ultrapure water were mixed in a 1.5 mL tube, followed by the addition of 2.5 μL of DNA Ligation Mix (Takara Bio; Cat. No. 6023) and incubation at 16°C for 30 min. .. The entire ligation mixture was transformed into 50 μL of DH5α competent cells (Takara Bio, Cat. No. 9057) and spread on LB agar plates containing 100 μg/mL ampicillin.

    Incubation:

    Article Title: A Water-Saving Drought Survival Phenotype in a Wheat TILLING Mutant Involves Survival-Biased Metabolic and Phosphorylation Reprogramming.
    Article Snippet: .. For TA cloning, 0.5 μL of T‐Vector pMD20 (Takara Bio Inc., Shiga, Japan; Cat. No. 3270), 0.5 μL of PCR product, and 1.5 μL of ultrapure water were mixed in a 1.5 mL tube, followed by the addition of 2.5 μL of DNA Ligation Mix (Takara Bio; Cat. No. 6023) and incubation at 16°C for 30 min. .. The entire ligation mixture was transformed into 50 μL of DH5α competent cells (Takara Bio, Cat. No. 9057) and spread on LB agar plates containing 100 μg/mL ampicillin.



    Similar Products

    97
    TaKaRa t vector pmd20
    T Vector Pmd20, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t+vector+pmd20/T-Vector+pMD+20/pm42809396-313-11-14
    Average 97 stars, based on 1 article reviews
    t vector pmd20 - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    97
    TaKaRa pmd20 plasmid
    Pmd20 Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t+vector+pmd20/T-Vector+pMD+20/pm41671091-697-29-31
    Average 97 stars, based on 1 article reviews
    pmd20 plasmid - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    97
    TaKaRa pmd20 t vector
    Pmd20 T Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t+vector+pmd20/T-Vector+pMD+20/10__1016_slash_j__jgr__2025__100974-83-8-10
    Average 97 stars, based on 1 article reviews
    pmd20 t vector - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    Image Search Results